Rapid lactic acid bacteria identification in dairy products by high-resolution melt analysis of DGGE bands

Comparisons of high resolution melt (HRM) profiles of known reference strains of LAB and their denaturing gradient gel electrophoresis (DGGE) bands showed very good agreement, allowing species recognition and identification from DGGE bands by HRM.

Use of HRM profiling for comparison and monitoring of total LAB communities from dairy products or starter cultures also can be evaluated, with a good agreement when comparing clustering of DGGE band profiles with clustering of HRM melting profiles.

In conclusion, identification of DGGE bands is possible by comparison of HRM melting profiles with known reference strains.

Original Source:

No comments:

Post a Comment